My Preliminary Findings

[fritolay66]

Since the time I had my fungal petri's examined, I had seen an entity then, that I thought was part of the fungal sample initially. There was also bacteria presnt in these cultures, but I didn't realize then, the involvement with the fungii. After many subsequent cultures, I have finally been successful in isolating the one thing that bothered me about the initial petri's and their evaluation, the bacteria. I have grown many now, and have watched these develop over and over again. I get the same results over and over again. And now, just recently, I have been able to watch the development of not only the bacteria in the first cultures, but the development of the same fungal entities, and their interactions.

I get the same three fungal entities over and over again, these are most likley contaminents. I get the same bacteria over and over again, and I can correlate these with what I see in the skin, the structures seen in the skin, and what I can grow in my agars, and what I see on slides. I have used varying agars, and done some simple lab tests on this bacteria. And here is what I have found.

1. It is absolutely gram positive.

A simple test.

Slideshow pics depicting gram positive:

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2. It is absolutely catalase positive.

A simple test.

Summer Fall 2011 Analysis VII :: H2O2.mp4 video by fritolay66 - Photobucket

3. It absolutely forms a mucoid colony.

Seen on agar, and in liquid agar.

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4. It is absolutely alpha-hemolytic in the first stages and progresses to
beta-hemolysis.

First 48 hours is alpha hemolytic on TSA blood agar.
From two days to 10 days, becomes progressively beta-hemolytic.
Day 10, most of blood agar is completely broken down.

Alpha and especially beta hemolysis are almost always pathogenic.

5. On TSA agar, it grows cream colored, mucoid colonies and is easily
transferred by loop. Appears brown under microscope.

6. It absolutely has a streptococcal-like formation in which the spherical form
will form chains.

Spherical- Has no cell wall or is not very well developed in early stages.
Morphs to mucoid cell wall and then capsule cell wall formation and/or
forms a capsule cell wall and forms additional mucoid cell surroundings with
an additional cell wall formation. I have seen both occur regularly.

Basic shapes and sizes, most commonly seen:

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C-shaped in Agar:

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Cell Wall:

Notice the scalloping of the edges as the sample ages.

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Enlarged photos of scalloping:

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7. Active mitosis is seen.

Slideshow pics for active mitosis:

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8. It is at least aerobic tolerant.

9. Flagella structures are seen.

Many of us have been tested by the Lyme Western Blot. There are many here and in the Lyme forums in which are only showing results in one band. That is the 41 kDa band. Flagella protein is just that, and this particular band I feel is cross reactive with the proteins of other flagellated bacteria other than Lyme. Although many experts will argue my position, in that the 41 kDa band is one of the earliest bands that will light up and is specific to Lyme keetes, and if clinically symptomatic is enough to diagnose and treat for Lyme. None the less, my result is my result, and whatever this test picked up, it has flagella.

Could have Lyme and other secondary infections. Yep, entirely possible, but what my petris are showing is why I question the result. And my petri's are showing a bacterial entity in which exhibits flagella like structures and pilli. And fimbrae, pilli, and flagella, all have cross reactive proteins.

10. Pilli structures are seen extending from bacteria to bacteria. These are
commonly seen in flagellated bacteria, and is a specialized pilli in which at
conjugation, plasmid information can be exchanged.

Slideshow for flagella and pilli

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11. I suspect it to produce H2S, Ammonia metabolites, and Calcium Oxalate.

12. I believe it uses melanin as a virulence factor, and elicits melanin
deposition in the skin.

13. I believe it prevents normal apoptosis of the epithelium.

14. It is absolutely capable of producing potent toxins.

15. Is synergistic with secondary fungal infections.

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16. It elicits cellular degranulation of histamine.

17. It elicits Janeway lesions.

18. It invades the mucous and epithelial tissues.

19. It uses and produces carotenoid pigments.

Interestingly enough, Carotenoids that give color to dietary fruits and vegetables are well recognized as potent antioxidants by virtue of their free radical scavenging properties and exceptional ability to quench singlet oxygen ( in the case of bacterial presence in the body, this would mean addressing oxidative chemicals used by the body to combat the bacteria). There are sources out there that have been exploring how pathogenic bacteria might take advantage of the antioxidant effects of carotenoid pigments to extend the bacteria's own lives, by inactivating chemicals deployed by neutrophils and macrophages that are lethal to most bacteria.

In GBS, the pore-forming ß-hemolysin/cytolysin (ßh/c) encoded by cylE is an important virulence factor as demonstrated in several in vivo models by these sources. In recent studies, these sources have sought to define the mechanism(s) by which both cylE-encoded phenotypes could contribute to GBS phagocyte resistance and increased virulence potential.

They found that cylE-deficient GBS was more readily cleared from a mouse's bloodstream, human whole blood, and isolated macrophage and neutrophil cultures. Survival of the bacteria was linked to the ability of ßH/C to induce cytolysis and apoptosis of the phagocytes.

I have talked about the "freakles" since day one of this nightmare. This is what I absolutely believe is taking place.

I have over 900 pic's of the tests I have performed. Slideshows and show and tell to follow next week as time allows.

Sample sent in for professional ident.

Frito

[jeanlong]

Hi fritolay66:

spectacular post. greatly appreciate the sharing of all this and your prior info.

Sure hope you continue to sort this out. Still working on it from the TCM approach here.

best regards
Jean

Originally Posted by fritolay66

Since the time I had my fungal petri's examined, I had seen an entity then, that I thought was part of the fungal sample initially. There was also bacteria presnt in these cultures, but I didn't realize then, the involvement with the fungii. After many subsequent cultures, I have finally been successful in isolating the one thing that bothered me about the initial petri's and their evaluation, the bacteria. I have grown many now, and have watched these develop over and over again. I get the same results over and over again. And now, just recently, I have been able to watch the development of not only the bacteria in the first cultures, but the development of the same fungal entities, and their interactions.

I get the same three fungal entities over and over again, these are most likley contaminents. I get the same bacteria over and over again, and I can correlate these with what I see in the skin, the structures seen in the skin, and what I can grow in my agars, and what I see on slides. I have used varying agars, and done some simple lab tests on this bacteria. And here is what I have found.

1. It is absolutely gram positive.

A simple test of which I have pics to document. Will post at later time.

2. It is absolutely catalase positive.

A simple test of which I have pics to document. Will post at later time.

3. It absolutely forms a mucoid colony.

Seen on agar, and in liquid agar.

4. It is absolutely alpha-hemolytic in the first stages and progresses to
beta-hemolysis.

First 48 hours is alpha hemolytic on TSA blood agar.
From two days to 10 days, becomes progressively beta-hemolytic.
Day 10, most of blood agar is completely broken down.

Alpha and especially beta hemolytic are almost always pathogenic.

5. On TSA agar, it grows cream colored, mucoid colonies and is easily
transferred by loop. Appears brown under microscope.

6. It absolutely has a streptococcal-like formation in which the spherical form
will form chains.

Spherical- Has no cell wall or is not very well developed in early stages.
Morphs to mucoid cell wall and then capsule cell wall formation and/or
forms a capsule cell wall and forms additional mucoid cell surroundings with
an additional cell wall formation. I have seen both occur regularly.

7. Active mitosis is seen.

8. It is at least aerobic tolerant.

9. Flagella structures are seen.

10. Pilli structures are seen extending from bacteria to bacteria. These are
commonly seen in flagellated bacteria, and is a specialized pilli in which at
conjugation, plasmid information can be exchanged.

11. I suspect it to produce H2S, Ammonia metabolites, and Calcium Oxalate.

12. I believe it uses melanin as a virulence factor, and elicits melanin
deposition in the skin.

13. I believe it prevents normal apoptosis of the epithelium.

14. It is absolutely capable of producing potent toxins.

15. Is synergistic with secondary fungal infections.

16. It elicits cellular degranulation of histamine.

17. It elicits Janeway lesions.

18. It invades the mucous and epithelial tissues.

I have over 900 pic's of the tests I have performed. Slideshows and show and tell to follow next week as time allows.

Sample sent in for professional ident.

Frito

[MeaganM]

Frito, from your research, do you think that it's possible that it can be treated with long term antibiotic medications or herbs? Have you tried to destroy the bacteria that you have cultured with different medias and if so, have you found any one thing that works better than another such as salt, menthol, etc...?

[Jo]

Wymore reported at Austin 2010 the bacterias he isolated to be:

E.coli, Nocardia, Staphylococcus, Streptococcus, Corynebacteria, Pseudomas, Clostridium

All were found in the control samples too, but the ones in bold were slightly elevated in morgellon samples.

Do you think your observations could fit Nocardia/Corynebacteria?
Nocardia - Wikipedia, the free encyclopedia

Clostridium is catalyse neg.

Jo

[sammy]

Megan, Long term antiboitics was what dr Harvey said and gave too his patients I do not think anyone here or I maybe wrong has been on anything long term . It is hard too get any doctor too admin this drug for a long period of time but my derm did put me on doxycline for 6 mos las week it seems frito is doing more research than anyone in the country. but what she is saying makes sense it certainly attacks the mucous and sinus and certainly the eyes sammy

[jeanlong]

These are generally meant to be used short term.

After completion a follow up with probiotics is often used to restore intestinal flora, equating to coaxing the internal environment and the body's own immune processes to encourage a stronger immune response.

found this article very interesting - low dose antibiotic in livestock feed is apparently creating new problems:

Our Big Pig Problem: Scientific American

[fritolay66]

Do you think your observations could fit Nocardia/Corynebacteria?
Nocardia - Wikipedia, the free encyclopedia

This one wouldn't surprise me, but I just don't know. There are so many parameters to this, that it is making it really tough to identify this on my own. Which is why I have sought the help of the big boys club. I can dink around with my simple testing all I want, but without professional back up, my results mean nothing, and also, some of my testing could very well be wrong, or there could be more than one bacteria in the culture, yadi ya. I needed the basic information to help me in causing some interest and elicit participation.

Frito, from your research, do you think that it's possible that it can be treated with long term antibiotic medications or herbs? Have you tried to destroy the bacteria that you have cultured with different medias and if so, have you found any one thing that works better than another such as salt, menthol, etc...?

Question 1: We shall see. When we can identify it, we can identify what it is sensitive to.

Question 2: Still currently performing these.

Frito

[fritolay66]

Adding show and tell to original post. Will take me the course of a week as I am right in the middle of my work week.

Frito

[skylark99]

Glued to this thread and holding my breath.

Thank you midwest wonder for all your hard work and diligence.

Jo, further to yours (and thanks!):

Nocard: rods, strict aerobes, not pleomorphic
coryne: rods, aerobic or facultatively anerobic, pleomorphic but rods with thickenings at ends, non-motile flagella

looks like we will know lots more shortly from the ident.

[fritolay66]

Guys,

I am so sorry but the financial strain of all this despite my working my *** off, is just too much. I am losing my internet today so I am told. I apologize for even starting this thread, but seriously, I just can't do it.

Take care. And again, I am so sorry. I am just simply struggling not to lose my home and eat.

Frito