Andy Coyles Important CDC Paper

[ladycolorado]

Carla had this CDC paper up on the shoutbox. It IS important enough to have its own thread and it should NOT get lost. She is the one who found it. Thanks Carla!

Photorhabdus Species: Bioluminescent Bacteria as Emerging Human Pathogens?

Here is Carla's IMPORTANT Find:
CDC - Photorhabdus Species: Bioluminescent Bacteria Emerging as Human Pathogens? .

(LC)

[carla]

Thanks Lady C ,
Thanks for this . It is a bit strange isn't it ?
But it wasn't my find .It is from Andy Coyle's work(where ever he may be now).I just thought it was time to look into it again .
He was very concerned about GM too which we now know he was right to be .

Andrew Coyle Petition/GLOBAL THREAT

[Sadsack]

Andy Coyle also mentioned this:

The scientific work that gets a Nobel Prize these days is often hard to understand and describe. But this year's chemistry award is a crowd pleaser: It went to one Japanese and two American scientists who made things glow in the dark — with a jellyfish gene.

Roger Tsien, a professor at the University of California San Diego; Martin Chalfie of Columbia University; and Osamu Shimomura, a Japanese researcher at the Marine Biological Laboratory in Woods Hole, Mass., were recognized for their work in advancing understanding of the machinery inside living cells.

Life is essentially an intricate dance of proteins inside every cell of our bodies — proteins that were too tiny to be seen.

So for decades, scientists have been trying to find a way to make that invisible world visible.

Fifteen years ago, Tsien tried attaching chemical dyes to a protein. It didn't work very well, and he thought what he really needed was a gene that makes the protein visible.

"Nobody knew of such a thing in the literature, but I sort of vaguely remembered that there was this protein thing called 'green fluorescent protein,'" Tsien says.

The protein came from a type of jellyfish and had been purified and described by Shimomura in the 1960s. It also glowed green when exposed to ultraviolet light.

Both Tsien and Chalfie tried inserting the gene that produced the protein into cells. No one knew if the technique would work, Tsien says, but "the amazing thing is that nature suddenly smiled."

The gene worked beautifully in bacteria, worms and lots of other creatures. Under ultraviolet light, it made proteins in the cell glow a ghostly shade of green. Thousands of researchers worldwide now use it to track proteins; they can watch cancer cells or viruses multiply and spread.

Tsien, Chalfie and Shimomura all got telephone calls from the Swedish Academy of Sciences early this morning.

Chalfie says he slept through his call. At a news conference later, he explained that he'd accidentally set his phone to ring very softly. When he got out of bed, though, he remembered it was the day the Nobel Prize in chemistry was to be announced.

"So I decided to find out who the schnook was that won it this year, so I opened up my laptop and found out that I was the schnook," Chalfie says. "The other two people are very good scientists."

Chalfie's research group was the first to insert the gene that produces the green florescent protein into another cell.

Tsien expanded the technique, creating an entire toolbox of glowing genes. He tinkered with the gene, creating dozens of new versions that glow in many colors. This allows researchers to tag different proteins with distinctive colors and observe their interactions.

"This is a practical Nobel Prize. This is something that has transformed medical research," says John Frangioni of Harvard Medical School.

"When we're able to cure terrible human diseases such as cancer and neurologic diseases, we're going to be able to trace that back to research that at some point used these fluorescent proteins," Frangioni says.

Tsien was thinking that maybe it was time to stop working on florescent proteins and move on to something new. But he says he recently discovered something else about them that's worth pursuing.

"The science calls," he says, "I can't quite punt this one away."

Jellyfish Protein Researchers Win Chemistry Nobel : NPR


SS

[Jo]

Good work ladies! - This bacteria is used to control fly pests.

I wonder what colour/s it glows?

Jo xxx

[carla]

OMG

The United States Environmental Protection Agency does not regulate insecticidal nematodes (Gorsuch, 1982), including genetically engineered strains (Andersen, personal communication).

OMFG

Regulatory constraints have hindered the development of some genetically engineered organisms. Insecticidal nematodes, however, possess a unique niche in the regulatory environment. In 1996, we readily obtained approval at federal, state and local levels to release our transgenic strain into turfgrass field plots at the Rutgers Upper Deerfield Experiment Station in Salem County, N.J. (4). Results from the study support the regulatory view that the transgenic nematode strain is an unlikely environmental threat.

OMFFFFFG

Now balance that with the statement made by Dr Randy Gaugler in his paper of 1996 at: http://www.aphis.usda.gov/brs/arthro.../9605201r.html

Which states:

APHIS has categorized H. bacteriophora as among “low-risk organisms which have already been released in the U.S.”

[carla]

Nancy and Steve might be interested in the jellyfish stuff he mentions.
Where is Nancy?? Has anyone heard from her?

[Baraka Obam]

I finally got time to read the article, this is a promising idea, but where is the testing. Will they have to take special measures to do the testing, If they don't take special measures, horse blood, temperature control, this and that, will they get lots of negatives. The area, the ankle is one of the worst for me, so could this be one of our dandy side afflictions or the vector, hmmmmm. The cumlative POSITIVE testing of strange emerging disease should be compiled and used on everyone that has unknown cause disease. I heard this from an OLD doctor long ago, he was looking for someone to talk with and told me how they decide the symtoms for disease by area of the country. He said they did a cumulative study of all the problems people were being tested for and then would compare them to actual disease syptoms of that area to get an approximation of the spread of certain disease. Then they would write a synopsis about tests to try first for simpler, quicker and better diagnosis. was this left behind because EVERYONE now has EVERYTHING, well, symptoms anyway.

[Kritters]

A very interesting find, Carls.

When I read this, some things just didn't seem right. See if you catch my drift. My synopsis below:
__________________________________________________
1) Photorhabdus is the only terrestrial bacteria known to exhibit bioluminescence
2) have never been known to live freely in soil
3) have only been isolated naturally from nematodes And the insects they parasitize
4) It seems likely therefore that Photorhabdus are transmitted to humans by a terrestrial invertebrate (nematode)
5) Agricultural scientists have been studying photorhabdus because of the role they play in controlling insects which LIKE HUMANS can be infested by nematodes
6) INSECT PATHOGENIC NEMATODES HARBORING PHOTORHABDUS ARE USED AS PESTICIDES IN A NUMBER OF COUNTRIES INCLUDING THE US AND AUSTRALIA ….AGRICULTURAL SCIENTISTS ARE ALSO ATTEMPTING TO DEVELOP INSECT-RESISTANT TRANS-GENIC CROPS BY USING INSECTICIDAL TOXIC GENES DERIVED FROM PHOTORHABDUS.
7) GENES ENCODING HOMOLOGUES OF INSECTICIDAL TOXINS FROM PHOTORHABDUS OCCUR NATURALLY WITHIN THE GENOME OF YURSINIA PESTIS…………..the cause of PLAGUE…………..lateral transfer of genetic material between photorhabdus and yursinia pesits is thought to have resulted from their common association with insects as bacterial pathogens.
(number 8...not smiley face ;-( The patients apparently acquired the pathogen from an unidentified source in the terrestrial environment.
__________________________________________________
My comments:

They’re saying Photorhabdus is the only terrestrial bioluminescent bacteria ….
Yet it has never been known to live freely in soil.

What am I missing? Is not ‘land’ actually ‘soil’? What’s ‘living freely’ in soil actually mean? Does it live in CONTAINED areas of soil?

So, it HAS been isolated naturally from nematodes and the insects which they ‘parasitize’…….but not from the soil or terrestria (is that a word?) or earth??? But the said nematodes come from and on the earth, no?

And they say in this report that it ‘seems likely’ photorhabdus is transmitted, therefore, by nematodes….(but not insects….because….the insects are parasitized and are not carrier? What about those which escape in time to bite a human?)

5—scientists have been studying them because of the role they play in controlling insects AND
6---they are not only studied, but actually USED as PESTICIDES AND TRANSGENIC CROPS through their toxic genes.

Not to mention….that the genes encoding homologues of said insecticidal toxins ALSO occur NATURALLY within the genome of the organisms which cause PLAGUE!

Oh….and the lateral transfer of genetic material between the two…they think results from COMMON ASSOCIATION OF INSECTS.

Sooo….the same insects which carry the plague carry photorhabdus, even though the latter isn’t transmitted by insects????

And finally…the patients acquired the pathogen from an UNIDENTIFIED source in the terrestrial environment. Sooooo that had to be a nematode, but not an insect?

Kritts

[Kritters]

Contrary to the CDC report, there are tons of terrestrial bioluminescent bacteria....If they're (according to the CDC) not usualy found on land but rather in science labs and the ocean, I think either a walking fish is transferring them to land, or ummm somehow they're coming from science labs.

Notice they are also used in sewage plants.

Bioluminescent……..bacteria (land and sea, and plenty of them), worms, FIREFLIES…BEETLES…ALGAE...mollusks (ABALONE IS A MOLLUSK)………all have luminescent properties.

"Luminescent plants and viruses do not exist….EXCEPT IN RECOMBINANT TECHNOLOGY."

Let’s face if folks, our Morgellons community incorporates possibly all of the above in our bodies.

Oh, CDC…do you have a Google feature you can use to research just how prevalent BIOLUMINESCENT BACTERIA IS IN THE TERRESTRIAL ENVIRONMENT? Who writes your papers for you…..a 3rd grader journalist for show and tell?




Access to articles : Nature Protocols

Grow Your Own Bioluminescent Algae

Bioluminescence - Chemistry Encyclopedia - Beetles/Fireflies, Dinoflagellates, Bacteria

Bioluminescence has been observed in many organisms and phyla throughout the terrestrial and aquatic worlds

Luminescent species are found among marine and terrestrial bacteria, annelids or segmented worms (e.g., fireworms), beetles (e.g., fireflies, click beetles, railroad worms), algae (e.g., dinoflagellates), crustaceans (e.g., shrimp, ostracod), mollusks (e.g., squid, clams, limpets), coelenterates (e.g., jellyfish, sea pansies, hydroids), bony fish (e.g., hatchet fish, flashlight fish, pony fish), and cartilaginous fish (e.g., sharks). Luminescent vertebrates (except for certain fish), mammals, higher plants, and viruses do not exist—except for those versions created by recombinant technology.
Beetles/Fireflies
Luciferases from click beetles, fireflies, and railway worms catalyze the ATP-dependent decarboxylation of luciferin (Figure 1). An AMP derivative of luciferin is formed, which subsequently reacts with O2. Cleavage of this dioxy derivative results in the emission of light characterized by wavelengths ranging from 550 nanometers (2.17 × 10−5 inches; green) to 630 nanometers (2.48 × 10−5 red, depending on the particular luciferase), and the release of CO2. Fireflies generally emit in the yellow to green range, as part of a courtship process; click beetles emit green to orange light; whereas railway worms emit red light, with green light being emitted on movement.
Bacteria
Bacterial luciferase catalyzes the reaction of reduced flavin mononucleotide (FMNH2) with O2 to form a 4a-peroxyflavin derivative that reacts with a long chain aldehyde leading to the emission of blue-green light (490 nanometers, or 1.93 × 10−5 inches) and the formation of riboflavin phosphate (FMN; the phosphorylated form of vitamin B2), H2O, and the corresponding fatty acid (Figure 3). Luminescent bacteria are found throughout the marine environment, living free, in symbiosis, or in the gut of marine organisms (including many fish and squid), as well as in the terrestrial environment as symbionts of nematodes.
ScienceDirect - Ecotoxicology and Environmental Safety : Validation of Genetically Engineered Bioluminescent Surfactant Resistant Bacteria as Toxicity Assessment Tools
Bio-engineered bioluminescent bacteria to terrestrial, wastewater, and brewery environvironments (Boyd et al.,1997). Nonpolar narcotics are assumed to produce their toxic ...
__________________________________________________ __

Amazing to me how they have the AUDACITY to report, in black and white, for all to read, how these bio-engineered creatures are being utilized for different purposes and they DON'T THINK they pose any danger to humans. DON'T THINK...not....POSITIVE THEY DO NOT. College students are even experimenting with this. And I love how they report new diseases and end the report with...'we don't know what causes it'.

Kritters

[carla]

Its all double speak like Andy Coyle pointed out somewhere in those post .All the jargon makes my head spin.

He was like a bull dog and would n't give up you know .I have no idea were he is now.
I m gonna post a few e-mails I got from him if I can find them.
xxxx